Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Basu, D.; Zhang, N.; Panigrahi, A.K.; Horton, T.M.; Pati, D.
    Development and validation of a fluorogenic assay to measure separase enzyme activity (2009), Anal. Biochem., 392, 133-138.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.49 additional information separase can be activated by incubating immunoglobulin G-Sepharose 6 containing ZZ TEV4-separase with anaphase-initiated Xenopus cytostatic factor to degrade its inhibitor securin at room temperature for 1 h Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.49 expressed in HEK-293T cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.49 securin
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.49 0.293
-
 acetyl-Asp-Arg-Glu-Ile-Nle-Arg-7-amido-4-methylcoumarin at 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.49 Rad21 + H2O Homo sapiens
-
 fragments of Rad21
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.49 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.49 HEL cell
-
 Homo sapiens
-
3.4.22.49 HL-60 cell
-
 Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
3.4.22.49 -20°C, 1 week, no significant loss of activity Homo sapiens
3.4.22.49 23°C, 48 h, 17% loss of activity Homo sapiens
3.4.22.49 4°C, 48 h, 12% loss of activity Homo sapiens
3.4.22.49 temperature, storage medium, duration, loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.49 acetyl-Asp-Arg-Glu-Ile-Nle-Arg-7-amido-4-methylcoumarin + H2O acetyl-Asp-Arg-Glu-Ile-Nle-Arg is the Rad21-peptidyl Homo sapiens acetyl-Asp-Arg-Glu-Ile-Nle-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.49 additional information does not cleave Leu-Glu-His-Asp-CHO-7-amido-4-methylcoumarin Homo sapiens ?
-
 ?
3.4.22.49 Rad21 + H2O
-
 Homo sapiens fragments of Rad21
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.49 37
-
-
 Homo sapiens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.22.49 30 55
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.49 4
-
 no appreciable activity is observed at 4°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.49 7.7
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.49 6 9
-
 Homo sapiens

Expression

EC Number Organism Comment Expression
3.4.22.49 Homo sapiens separase is overexpressed and mislocalized in a wide range of human cancers, including breast, prostate, and osteosarcoma up

General Information

EC Number General Information Comment Organism
3.4.22.49 physiological function separase plays a pivotal role in the separation of sister chromatids at anaphase by cleaving its substrate cohesin Rad21 Homo sapiens